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Home Page | Add to Favorites | Print Page | Best Viewed in Mozilla Firefox (1024X768) | Steps of DNA ReplicationThis page summarizes the process of DNA replication in eukaryotic cells, by dividing it into simpler steps.Note: You may click on some parts of the images to get a better description. Step 1
Step 2 1) SSBs bind to the exposed DNA strands and block hydrogen bonds, therefore maintaining the separation between the two strands. 2) DNA Gyrase relieves tension from the unwounded strands by cutting the DNA strands, and then resealing them. *Not shown on diagram.
Step 3 1) RNA Primase attaches RNA nucleotides which bind to the DNA nucleotides of the 3'-5' strand (original). RNA nucleotides are the “primers” for the binding of DNA nucleotides. These primers must be present in order for DNA synthesis to occur.
Step 4 1) At this point there are two strands: • Leading (5’-3’): DNA Polymerase III "reads" the template strand and continuously adds nucleotides (complementary to the nucleotides of the template) • Lagging (3’-5’): This template cannot be "read" by DNA Polymerase III. In the lagging strand the RNA Primase adds additional RNA Primers so that the DNA polymerase III reads the template but lengthens it in bursts. The fragments between any two RNA primers in the lagging strand are called "Okazaki Fragments".
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