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DNA Replication
What is DNA?
Mitosis
DNA Replication and Repair
Steps of DNA Replication
Enzymes of DNA Replication
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Steps of DNA Replication

This page summarizes the process of DNA replication in eukaryotic cells, by dividing it into simpler steps.
Note: You may click on some parts of the images to get a better description.

Step 5
1) In the lagging strand the DNA Polymerase I “reads” the newly formed fragments by DNA Polymerase III and removes the existing RNA Primers. The gaps are closed by the addition of complementary nucleotides.

DNA Polymerase III
Leading Strand
SSBs
DNA Helicase
DNA Polymerase I
Lagging Strand
Okazaki Fragments
Okazaki Fragments
Direction of Replication Okazaki Fragments
Okazaki Fragments



Step 6
1) DNA Ligase follows DNA Polymerase I, and joins the Okazaki fragments into one strand by adding phosphates in the remaining – phosphodiester bonds.
2) DNA Polymerase reaches to an end of the strands. When the last RNA primer is removed, it is not possible for the DNA Polymerase I to seal the gap. Therefore, the end of the original strand where the last primer binds is not replicated. These ends consist of non-coding DNA that contains repeated sequences which are called telomeres.
3) Exonuclease searches the replicated strands for any errors and fixes them by replacing the wrong nucleotides. A part of the telomeres is removed as well.

DNA Polymerase III
Leading Strand
SSBs
DNA Helicase
Nucleotides Lagging Strand
DNA Ligase
Direction of Replication



Step 7
1) DNA replication is completed, and a newly formed strand can now be used as the genetic material for a new cell which is created by mitosis.

New Strand
Original Strand

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